mt2 antibodies  (Alomone Labs)

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Expression of MT1 and MT2 genes in rat TG, SPG and hypothalamus. ( A ) Comparison of MT1 and MT2 mRNA levels in the TG of male and female rats shows that MT1 expression is significantly higher than MT2. ( B ) In both the hypothalamus (HT) and SPG of male rats, MT1 mRNA levels are also significantly higher than MT2. Data are presented as mean ± SEM, statistical significance indicate as *** p < 0.001, ** p < 0.01, * p < 0.05, and n = 6

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Expressing, Comparison

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: MT1 and MT2 immunoreactivity in TG. ( A ) positive MT1 immunoreactivity was observed in the cytoplasm and nuclei of TG neurons (thick arrows) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was observed predominantly in the cytoplasm of neurons (thick arrows), in some cases in their nuclei (thick arrows), and in Aδ-fibers (thin arrows). Negative cells are indicated by asterisks in both ( A ) and ( B ). Blue color represents nuclei staining with DAPI. ( C ) Bar graphs show the number of MT1 and MT2 immunoreactive TG neurons in male and female rats. There was no significant difference in MT1 or MT2 protein expression between sexes. However, the number of MT1-immunoreactive cells was approximately twofold higher than that of MT2 in both males and females. Data are presented as the mean ± SEM, n = 6 and * p < 0.05

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Staining, Expressing

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Double immunohistochemistry of MT2 with CGRP or RAMP1 in TG. ( A and D ) MT2 immunoreactivity (green fluorescence) was observed in cytoplasm of TG neurons (thick arrows), and in Aδ- fibers (arrowheads). ( B ) CGRP (red fluorescence) was expressed in small to medium-sized TG neurons (thick arrows) as well as in C-fibers (arrowhead). ( C ) MT2 co-localized with CGRP (yellow-orange) in the cytoplasm of small to medium-sized neurons (thick arrows). ( E ) RAMP1 (red fluorescence) was expressed in the cytoplasm of medium to large- sized TG neurons (thick arrow) and in Aδ-fibers (arrowhead). ( F ) The merged imaged showed that MT2 was co-localized (yellow) with RAMP1 in the cytoplasm of medium to large-sized neurons (thick arrow) as well as in the Aδ- fibers (arrowhead)

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Immunohistochemistry, Fluorescence

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Double immunohistochemistry of MT2 and CASPR in trigeminal Aδ-fibers. ( A ) MT2 receptors (green) were found in the Aδ-fibers. ( B ) CASPR immunoreactivity (red) was used to label the paranodal regions of nodes of Ranvier. ( C ) MT2 and CASPR were co-expressed in Aδ-fibers. The blue color represents nuclei staining with DAPI

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Immunohistochemistry, Staining

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Double immunohistochemistry of MT2 with CASPR and CGRP in rat dura mater. ( A and D ) Thin MT2-immunoreactive axons (green, arrows) were observed in the rat dura mater. ( B ) These fibers were identified as Aδ-fibers based on CASPR immunoreactivity (red) showing the characteristic bowtie-shaped paranodal pattern (arrowheads). ( A - C ) Fiber bundles were often seen flanking dural blood vessels (asterisks). ( C ) MT2 and CASPR were observed to be co-expressed in the same Aδ-fibers. ( E ) CGRP immunoreactivity (red, arrowheads) was detected in c-fibers throughout the rat dura mater in a granular, pearl-like pattern. ( F ) Merged images show intertwined C- and Aδ-fibers, expressing CGRP and MT2 respectively, projecting throughout the dura mater

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Immunohistochemistry, Expressing

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Localization of MT2 immunoreactivity in the SPG and its co-localization with VIP and PACAP. ( A , D and G ) MT2 immunoreactivity (green) was observed in the cytoplasm of neurons (thick arrows), and in fibers (arrowhead). ( B and E ) VIP immunoreactivity (red) was detected in the cytoplasm of many medium-sized neurons (thick arrows) and in a few fibers (arrowheads). ( C and F ) MT2 was co-localized with VIP in the cytoplasm of medium sized neurons (yellow-orange, thick arrows). D , E and F are higher-magnification views of images A , B and C , respectively. ( H ) PACAP immunoreactivity (red) was found in the cytoplasm of SPG neurons (thick arrows). ( I ) Double staining of MT2 and PACAP showed that MT2 was co-localized with PACAP in the cytoplasm of SPG neurons (yellow-orange, thick arrow)

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Double Staining

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: MT1 and MT2 immunoreactivity in the DRG. ( A ) MT1 expression in DRG was observed in both the cytoplasm and nuclei of neurons (thick arrow) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was localized in the cytoplasm of neurons and in some cases in their nuclei (thick arrow), and in the Aδ-fibers (thin arrows). The blue color represents nuclei staining with DAPI

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Expressing, Staining

Journal: Translational Psychiatry

Article Title: Melatonin-induced ApoE expression in mouse astrocytes protects endothelial cells from OGD-R induced injuries

doi: 10.1038/s41398-020-00864-9

Figure Lengend Snippet: a Western blot showing ApoE protein level in the lysate of astrocytes treated with OGD-R and melatonin at indicated concentrations (0, 0.001, 0.1, 1, 10, and 100 nM). b CCK-8 assay showing the cell viability of co-cultured endothelial cells treated with OGD-R, melatonin (1 nM) or RAP (200 nM; n = 4). c ROS production in co-cultured endothelial cells treated with OGD-R, melatonin (1 nM), or RAP (200 nM; n = 4). d Caspase-Glo 3/7 assay showing caspase activity in co-cultured endothelial cells treated with OGD-R, melatonin (1 nM), or RAP (200 nM; n = 4). e Apoptosis rate revealed by Annexin V/PI assay in co-cultured endothelial cells treated with OGD-R, melatonin (1 nM), or RAP (200 nM; n = 4). f Western blot showing the protein levels of melatonin receptors (MT1 and MT2) in HUVEC cell or astrocytes. g CCK-8 assay showing the cell viability of endothelial cells treated with OGD-R or melatonin (1 nM; n = 4). h ROS production in endothelial cells treated with OGD-R or melatonin (1 nM; n = 4). i Apoptosis rate revealed by Annexin V/PI assay in endothelial cells treated with OGD-R or melatonin (1 nM; n = 4). j Western blot showing ApoE protein levels in the lysate and medium of astrocytes treated with melatonin (1 nM) or 666-15 (0.1 μM). k Real-time PCR showing ApoE mRNA levels in astrocytes treated with melatonin (1 nM) or 666-15 (0.1 μM). ** P < 0.01, *** P < 0.001.

Article Snippet: Following antibodies were used: ApoE antibody from abcam (ab1907), LRP1 antibody from abcam (ab92544), LRP8 antibody from abcam (ab108208), LDLR antibody from abcam (ab52818), MT1 antibody from alomone (AMR-031), MT2 antibody from alomone (AMR-032), Akt antibody from CST (4691), Phospho-Akt (Ser473) antibody from CST (9271), eNOS antibody from abcam (ab76198), and Phosphor-eNOS (S1177) antibody from abcam (ab215717).

Techniques: Western Blot, CCK-8 Assay, Cell Culture, Caspase-Glo Assay, Activity Assay, Real-time Polymerase Chain Reaction